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. 2018 Mar 22;12:79. doi: 10.3389/fncel.2018.00079

Figure 3.

Figure 3

TDP-43 mutationabolished its nuclear loss induced by ICH. (A) Western blotanalysis of TDP-43 and P-TDP-43 protein levels after exposureto the TDP-43 plasmid mutation in the whole cell lysate, nucleus and cytoplasm. (B) P-TDP-43 protein levels were normalized to that of β-tubulin. (C) P-TDP-43 protein levels were normalized to that of TDP-43. (D) TDP-43 protein levels were normalized to that of β-tubulin. (E) TDP-43 protein levels in the nucleus were quantified, and the mean values of TDP-43 protein levels in the sham group were normalized to 1.0. Histone H3 served as the loading control. (F) TDP-43 protein levels in the cytoplasm were quantified, and the mean values of TDP-43 protein levels in the sham group were normalized to 1.0. β-tubulin served as a loading control. All values are mean ± SEM, *p < 0.05 vs. sham; **p < 0.01 vs. ICH + empty vector; ###p < 0.001, ##p < 0.01, #p < 0.05 vs. ICH + empty vector; @@p < 0.01, @p < 0.05 ICH + TDP-43 plasmid vs. ICH + TDP-43 plasmid mutation; &p < 0.05 ICH + siRNA vs. ICH + control RNA. n = 6. (G) Immunofluorescence staining to assess distribution of TDP-43 after exposure to the TDP-43 plasmid mutation, and the brain region used for the slides was between the cerebral cortex and the perihematomal, scale bar: 50 μm.