FIG 6.
Intracellular vRdRp assays and cytotoxicity study. (A) Raw CT values of mRNA transcripts from the minigenome assay and reporter gene, β-actin. Each CT value is taken as an average of three qPCRs, and error bars represent the standard deviations. (B) Normalized ΔCT values to the Mock, set as 1. Each ΔCT was calculated by subtracting the experimental CT value to its subsequent β-actin CT, and the error bars represent the standard deviations. This shows a significant decrease in mRNA transcripts when UMSL1011 is added. ***, P < 0.001; ns (not significant), P > 0.05. (C) Cytotoxicity studies. Monolayers of HeLa cells were incubated with 130 and 650 μM UMSL1011, respectively, for 25 h. We used 1% DMSO as the solvent control. Cell viability was measured using MTT assays. Experiments were carried out in triplicate, and error bars represent the standard deviations.