FIG 3.

Surface residues of the P2 domain of FCV-5 VP1 were selected for site-directed mutagenesis in the recombinant FCV-5–Urbana clone. (A) Surface view of the P2 dimer of FCV-5 VP1 protein. Residues targeted for mutagenesis in the recombinant Urbana-FCV-5 clone are labeled with circles. Degree of conservation of surface-exposed residues on the P2 subdomain as predicted by Consurf 3.0. The solid yellow line indicates the dimer interface. (B) fJAM-A contact residues on the surface of the VP1 dimer predicted from the FCV-F9-fJAM-A cryo-EM structure (25) are highlighted in yellow. (C) An alignment of the amino acid sequences within the P2 domain of FCV-5 and FCV-Urbana. Residues 475 to 521 are shown. Nonidentical residues selected for mutagenesis are indicated.