TABLE 1.
Residues on the surface of the P2 subdomain of VP1 chosen for mutagenesis
| Residue in FCV-5 | Mutation | Conserved or variable | fJAM-A binding sites predicted by cryo-EMa |
|---|---|---|---|
| Residues differing between FCV-5 and FCV-Urbana | |||
| N478 | Db | Conserved | No |
| V481 | Ib | Variable | No |
| K493 | Eb | Variable | Yes |
| R518 | Db | Variable | No |
| Conserved residues | |||
| P431 | A | Conserved | No |
| G433 | A | Conserved | Yes |
| I437 | A | Conserved | Yes |
| A454 | S | Conserved | Yes |
| N459 | A | Conserved | No |
| N462 | A | Conserved | Yes |
| K479 | A | Conserved | Yes |
| T484 | A | Conserved | No |
| D513 | A | Conserved | No |
| H515 | A | Conserved | Yes |
a
Contact residues of VP1 were identified following fitting experiments of vaccine F9 strain with fJAM-A by cryo-EM (25).
b
These residues correspond to amino acids in FCV-Urbana at that position.