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Cleavage of candidate substrates in poliovirus-infected HeLa cells in the presence of zVAD-FMK. HeLa cells were infected with poliovirus (MOI of 10) in the presence of DMSO or 50 μM zVAD-FMK (7 hpi). Candidate proteins were detected by immunoblotting using the indicated antibody. N, N-terminal cleavage product; C, C-terminal cleavage product.
