FIG 8.

Candidate substrates identified by TAILS modulate poliovirus infection. HeLa cells were transfected with either a scrambled (siSCX) or a candidate-specific siRNA (si- prefix) for 24 to 72 h, followed by poliovirus infection (MOI of 0.1) for 7 h. Titers of extracellular and intracellular virus were determined by plaque assay, and titers were calculated as the number of PFU per milliliter from at least three independent experiments. (*, P < 0.05; **, P < 0.01). ND, no statistically significant difference. Western blots of the indicated proteins are shown below.