TABLE 1.
Summary of total peptide and proteins identified by TAILS analysis of poliovirus and CVB3 3Cpro
| 3Cpro and cell type | Instrument | No. of expts | Total no. of peptides | No. of unique peptides |
No. of unique proteins |
No. of high-confidence substrates |
|---|---|---|---|---|---|---|
| PV 3Cpro in HeLa cell lysates | ||||||
| OrbiXL | 3 | 627 | 534 | 444 | 10 | |
| QExactive | 2 | 1,778 | 1,741 | 1,059 | 37 | |
| QToF | 2 | 1,077 | 759 | 614 | 10 | |
| Orbi + QExactive | 5 | 2,253 | 2,224 | 1,239 | 15 | |
| Total for the group (unique) | 7 | 3,482 | 3,210 | 1,965 | 72 | |
| CVB 3Cpro in HL-1 cell lysates | QExactive | 3 | 364 | 347 | 240 | 34 |