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Depletion of EGFR expression enhanced the levels of several ISGs. IPEC-J2 (A) and HEK293 (B) cells were transfected with control siRNA or EGFR-specific siRNA for 24 h. Total RNA was extracted, and the mRNA levels of MxA, ISG15, and IFN-β were determined by quantitative RT-PCR. The results are representative of three independent experiments (means and SD). *, P < 0.05. The P value was calculated using Student's t test.
