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PMCA conversion coefficient of hydrated hamster PrPSc is reduced after 500 cycles of freezing and thawing when bound to soil. Selected samples, untreated control (F.T. 0), and samples treated with 500 cycles of freezing and thawing (F.T. 500) were subjected to 10-fold serial dilutions followed by one round of PMCA (two rounds for SCL-adsorbed DY). The conversion coefficient of samples treated with 500 cycles of freezing and thawing was compared to that of untreated controls.
