TABLE 2.
Preparation of soil-adsorbed HY TME, DY TME, and CWD PrPSca
| Soil/soil mineral | Incubation time (h) | Soil (mg soil/ml solution) | PrPSc (mg brain equivalent/ml solution) | Soil resuspension (mg soil/μl solution) | PMCA seed (mg brain equivalent/100 μl substrate) |
|---|---|---|---|---|---|
| Rinda SCL | 24 | 5 | 5b/10c | 0.1 | 0.1b/0.2c |
| Bentonite clay | 24 | 5 | 5 | 0.1 | 0.1 |
| SiO2 powder | 24 | 50 | 2.5 | 0.5 | 0.1 |
a
SCL-adsorbed DY TME and CWD were prepared with the same soil-to-brain ratio and under the same conditions as SCL-adsorbed HY TME.
b
Value for 10% brain homogenate of HY TME, DY TME, and CWD363.
c
Value for 20% brain homogenate of CWDt01821.