FIG 2.

Spherule location and replication activity. At 4 h p.i., PNS samples were prepared from SFV-infected (MOI of 500) BHK cells, treated with 100 nM wortmannin at 1.5 h p.i. or 5 μM nocodazole at 0 h p.i., or left untreated. These samples were compared to the PNS samples prepared from SFV-infected (MOI of 50), untreated cells to give the percentages indicated in panels A to C. Error bars represent standard deviations from two independent experiments. (A and B) Total RNA was isolated from the PNS samples and plus- and minus-strand RNA were detected by in-gel hybridization with specific probes, followed by quantification of 42S RNA. (C) In vitro incorporation of [³²P]CTP into viral RNA. 42S RNA was quantified.