FIG 2.

Infection of CPEpiC and HMC is sialic acid dependent. (A) Cells were either untreated or treated with type II neuraminidase to remove cell surface sialic acid receptors. The cells were then incubated with Alexa Fluor 488-labeled JCPyV and analyzed by flow cytometry. The histograms are shown in the insets. (B) This same treatment inhibits JCPyV infection of all three cell types as indicated. Error bars represent standard errors of the mean for three independent experiments performed in triplicate.