FIG 5.

Vaccine induction of broad V1V2 binding antibody responses. (A) Macaque plasma IgG binding to gp70 B.CaseA2 V1V2 was examined before vaccination (week 0) or from the weeks of SHIV challenge using the BAMA. SHIV challenges occurred weekly beginning at week 84. Results for each vaccinated group are shown in individual graphs, with each curve representing an individual macaque. Open circles and filled circles represent time points where the macaque was uninfected and infected, respectively. (B and C) Comparison of plasma IgG binding magnitude as background subtracted median fluorescence intensity (B) or area under the curve (C) for response to gp70 B.CaseA2 V1V2 among the three groups. Each column represents one vaccinated group, and individual macaques are shown by each symbol. Nonresponders are shown by open black symbols; animals were considered nonresponders if their responses were below 100 and less than three times background. There were no significant differences observed among vaccinated groups. (D) Group median fluorescence intensity (median fluorescence intensity minus background) for week 84 plasma IgG binding to 19 V1V2 constructs. Each symbol represents the group median fluorescence for a unique V1V2 construct. The middle line of the box shows the median binding for all 19 constructs, and the whiskers represent the maximum and minimum median values. (E) Comparison of the magnitudes of binding by week 84 plasma IgG to the 19 V1V2 constructs listed across the top. Each row shows the binding magnitudes for the immunogen shown on the left. Each column shows a different V1V2 antigen. The squares are the median binding magnitude (median fluorescence intensity minus background) for each V1V2 construct for an entire group. The binding titers are color-coded as indicated on the right.