FIG 3.

Presence of p24⁺ α₄β₇⁺ T_CM in HIV-1-infected α₄β₇⁺ T_EM. α₄β₇⁺ T_EM were infected with HIV-1_BJZS7 in the presence or absence of 1 μM maraviroc (Mara). The phenotypes of the cells were analyzed by flow cytometry. (A) Representative results show the expression of p24 versus CD4 (i) or CCR5 (ii) on CD3⁺ CD8⁻ T cells 6 days after infection (donors = 5). (B) (i) Representative flow cytometric analysis shows that CCR7 expression on uninfected and infected α₄β₇⁺ T_EM was upregulated at 6 days after infection (donors = 5). (ii) Percentage of p24⁺ α₄β₇⁺ T_EM and p24⁺ α₄β₇⁺ T_CM in CD3⁺ CD8⁻ T cells (donors = 5). (iii) Representative histograms show the expression of CCR5 on uninfected, p24⁻, and p24⁺ α₄β₇⁺ T_CM (CCR7⁺) or α₄β₇⁺ T_EM (CCR7⁻) (donors = 5). The shaded histogram represents the isotype control. Data are expressed as the mean ± SEM. **, P < 0.01.