FIG 6.
Effect of TGF-β on α4 and β7 expression on α4β7+ TEM and binding toward MAdCAM-1. α4β7+ TEM were isolated and cultured in the presence of 1 ng/ml or 10 ng/ml TGF-β for 7 days. (A) The effect of TGF-β on the percentage (i) and the MFI (ii) of α4 and β7 expression on CD4+ CD45RO+ T cells was determined on day 7 (donors = 5). (B) The effect of TGF-β on the total percentage of MAdCAM-1–Fc binding cells and the binding of MAdCAM-1–Fc on α4β7+ TEM and α4β7+ TCM was determined on day 7 (donors = 3). Data are expressed as the mean ± SEM. *, P < 0.05; **, P < 0.01.