FIG 2 .

Role of TFEB in A. baumannii internalization by A549 cells. (A and C) Immunoblot analysis of A549 cells transfected with scrambled (SC) and TFEB siRNA and pEGFP-N1-TFEB for 48 and 24 h, respectively. Values in the bar graphs are the percentages of TFEB level in control (CTL) and transfected A549 cells. (B, D, and E) A549 cells were transfected with SC and TFEB siRNA and pEGFP-N1-TFEB and infected with 10⁸ CFU/ml A. baumannii ATCC 17978 for 2, 4, or 8 h. An assay of adherence and invasion of A. baumannii ATCC 17978 into A549 cells was performed as described in Materials and Methods. The effect of TFEB siRNA and pEGFP-N1-TFEB mediated TFEB depletion and overexpression, respectively, on adherence or invasion of A. baumannii ATCC 17978. The percentages of total nontransfected A549 cells and A549 cells incubated with A. baumannii ATCC 17978 are shown for both adhesion and invasion. Results are from three independent experiments, and data are the means plus SEM (error bars). Values for untransfected and transfected groups in panels B and E that are significantly different (P < 0.05) are indicated by an asterisk. Values in panel E that are significantly different (P < 0.05) are indicated by bars and asterisks as follows: **, ATCC 17978 cells transfected with siRNA TFEB and ATCC 17978 cells or ATCC 17978 cells transfected with pEGFP-N1-TFEB; ***, ATCC 17978 cells and ATCC 17978 cells transfected with pEGFP-N1-TFEB.