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. 2018 Mar 22;9:430. doi: 10.3389/fmicb.2018.00430

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Copyright © 2018 Gong, Dai, Zhang, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course analysis of transcript levels of genes encoding (A) transcription activator (xlnR; g3648.t1), (B) D-xylose reductase (xyrA; g219.t1), (C) L-arabitol dehydrogenase (ladA; g1617.t1), (D) xylulose reductase (lxrC; g3669.t1), and (E) xylitol dehydrogenase (xdhA; g3399.t1) by qPCR in Aspergillus niger An76 induced using 1% xylose or 1% XOS. Glyceraldehyde-3-phosphate dehydrogenase (gapdh; g7576.t1) was used as reference gene. The results were calculated as the relative transcription level using the 2^−ΔΔCt method. Values represent the mean from two independent biological experiments measured in triplicate, and error bars indicate the standard deviations.