Table 1.
The expression of xylan-degrading enzymes secreted by Aspergillus niger An76 on day 5 of growth on different carbon sources.
| Activity | Gene name | Family | Genome ID | PSM ± sd | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glycerol | Glucose | Cellobiose | Xylose | XOS | Xylan | Arabinose | Xylitol | CMC | |||||
| XYLAN-BACKBONE DEGRADATION ENZYMES | |||||||||||||
| Xylanase | Endo-1,4-beta-xylanase | xynC | GH10 | g1233.t1 | 4 ± 2.0 | 20 ± 5.1 | 15 ± 6.4 | 86 ± 15 | 73 ± 16 | 78 ± 15 | 0 ± 0.0 | 21 ± 3.4 | 87 ± 9.0 |
| xynB | GH11 | g10033.t1 | 4 ± 2.0 | 30 ± 5.0 | 21 ± 6.0 | 301 ± 29 | 409 ± 30 | 360 ± 40 | 36 ± 7.0 | 69 ± 10 | 146 ± 15 | ||
| xynA | GH11 | g9709.t1 | 0 ± 0.0 | 2 ± 0.0 | 0 ± 0.0 | 73 ± 19 | 84 ± 24 | 139 ± 16 | 0 ± 0.0 | 84 ± 4.0 | 20 ± 4.0 | ||
| β-xylosidase | xlnD | GH3 | g662.t1 | 21 ± 5.7 | 1 ± 0.0 | 9 ± 2.7 | 153 ± 22 | 151 ± 22 | 543 ± 32 | 98 ± 12 | 61 ± 11 | 42 ± 7.0 | |
| xlnB | GH43 | g4894.t1 | 0 ± 0.0 | 7 ± 3.3 | 23 ± 3.5 | 9 ± 2.8 | 3 ± 0.98 | 4 ± 2.2 | 1 ± 0.63 | 0 ± 0.0 | 12 ± 3.1 | ||
| XYLAN ACCESSORY ENZYMES | |||||||||||||
| Arabinan endo-1,5-α-L-arabinosidase | abnA | GH43 | g8588.t1 | 2 ± 0.4 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 4 ± 1.4 | 0 ± 0.0 | 0 ± 0.4 | |
| Endo-arabinase | abnB | GH43 | g3804.t1 | 4 ± 1.4 | 128 ± 10 | 106 ± 7.0 | 90 ± 8.0 | 29 ± 8.0 | 6 ± 1.3 | 11 ± 2.8 | 0 ± 0.0 | 87 ± 4.3 | |
| Arabinoxylan arabinofuranohydrolase | axhA | GH62 | g1234.t1 | 27 ± 7.5 | 124 ± 11 | 76 ± 12 | 161 ± 26 | 432 ± 32 | 156 ± 11 | 169 ± 11 | 73 ± 6.0 | 76 ± 9.3 | |
| a-L-arabinofuranosidase | abfA | GH51 | g10075.t1 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 18 ± 6.0 | 0 ± 0.0 | 0 ± 0.0 | |
| abfB | GH54 | g3885.t1 | 42 ± 7.0 | 8 ± 3.3 | 7 ± 2.3 | 166 ± 23 | 125 ± 18 | 78 ± 13 | 723 ± 33 | 60 ± 7.3 | 20 ± 3.3 | ||
| abfC | g4219.t1 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 1 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | 0 ± 0.0 | |||
| β-xylosidase: a-L-arabinofuranosidase | abfD | GH43 | g4234.t1 | 4 ± 1.6 | 0 ± 0.0 | 8 ± 1.1 | 10 ± 2.7 | 22 ± 4.3 | 22 ± 3.8 | 2 ± 1.3 | 3 ± 2.0 | 16 ± 3.4 | |
| a-glucuronidase | aguA | GH67 | g5372.t1 | 10 ± 1.6 | 4 ± 1.7 | 19 ± 2.0 | 92 ± 8.4 | 94 ± 5.4 | 135 ± 10 | 14 ± 3.1 | 22 ± 2.8 | 21 ± 2.5 | |
| Feruloyl esterase | faeA | g7596.t1 | 8 ± 2.1 | 0 ± 0.0 | 7 ± 1.8 | 4 ± 0.75 | 2 ± 0.8 | 4 ± 1.9 | 2 ± 1.1 | 3 ± 1.0 | 0 ± 0.0 | ||
| faeB | g10211.t1 | 6 ± 1.7 | 0 ± 0.0 | 10 ± 2.1 | 6 ± 2.3 | 6 ± 1.0 | 1 ± 0.5 | 5 ± 1.6 | 0 ± 0.0 | 9 ± 2.8 | |||
| Acetylxylan esterase | axeA | g7679.t1 | 3 ± 0.8 | 0 ± 0.0 | 4 ± 1.2 | 38 ± 6.6 | 141 ± 15 | 30 ± 9.0 | 5 ± 1.4 | 6 ± 1.1 | 15 ± 2.9 | ||
PSM, Peptide Spectrum Matches; sd, standard deviation.