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. 2001 Sep 18;98(20):11423–11427. doi: 10.1073/pnas.211303598

Figure 1.

Figure 1

Detection of the genes overexpressed in HP1-null larvae [04/05(−/−)] relative to wild type [WT(+/+)] by RDA. (A) Ethidium bromide-stained agarose gel showing difference products generated by PCR-amplification after the first (Dp1), second (Dp2), and third (Dp3) rounds of subtractive hybridization in RDA. Arrows indicate prominent bands of Dp2 and Dp3. DNA ladder indicates 100-bp differences, and the 0.5-kb band (0.5 kbp) is indicated. (B) Northern blot analysis of RNA from HP1-null and wild-type larvae with radiolabeled Ser4 and CG13135 cDNA fragments isolated from Dp2 and Dp3. 04/05(−/−) represents HP1-null larvae generated by crossing between heterozygous Su(var)2–504 and Su(var)2–505 parents.