Figure 2.

Expression of Ser4 and CG13135 is regulated by HP1. (A) Northern blot analysis of Ser4 and CG13135 RNA in larvae with different HP1 heterozygous (+/−) or null (−/−) mutant backgrounds. (B) Comparative intensities of bands are normalized to wild type (WT) and expressed in percentages. Bars indicate standard errors from three independent experiments. 04/05(−/−) and 04/05(−/+) larvae were generated from Su(var)2–5⁰⁴/CyO, y⁺ and Su(var)2–5⁰⁵/CyO, y⁺ parents. 04/149(−/−) and 04/149(−/+) were produced by crossing between Su(var)2–5⁰⁴/CyO, y⁺ and Su(var)2–5¹⁴⁹/CyO, y⁺ parents. Transcripts of HP1 and Rp49 are shown as loading controls. Note that these Su(var)2–5 alleles have no effect on steady-state HP1 RNA message but yield no normal HP1 protein (refs. 3 and 5).