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. 2018 Mar 22;12:81. doi: 10.3389/fncel.2018.00081

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Copyright © 2018 Pischedda, Montani, Obergasteiger, Frapporti, Corti, Rosato Siri, Volta and Piccoli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biochemical analysis of cryopreserved cultures. (A) We analyzed the expression level of specific synaptic markers in acutely dissociated cortical neurons vs. cultures cryopreserved for 2 or 4 weeks. Cultures were processed for western-blotting at DIV14 to measure synapsin, MAP2, GluA1 and β-actin levels. (B) The graph reports synapsin, MAP2 and Glu1A levels, expressed as relative optical density (ROD) normalized vs. β-actin. Data are expressed as mean ± SEM, n = 6. (C) The scatter plots show the distribution of single data points obtained from acutely dissociated (black), 2-weeks (green) and 4-weeks (red) cryopreserved cultures coming from two distinct neuronal preparations (prep A and prep B) or combining data from three fresh cultures A (circle), B (square) and C (triangle; fresh A + B + C).