Table 1.
In vitro reducing ability (RA %) in DPPH assay, soybean lipoxygenase inhibition (% LOX inhbt) and anti-lipid peroxidation activity (A-LP %).
| Compound | RA # % ± SD, DPPH, (20 min) | RA # % ± SD, DPPH, (60 min) | % LOX ± SD Inhbt @ (0.1 mM) | A-LP % ± SD @ (0.1 mM) |
|---|---|---|---|---|
| 1 | 84 ± 1.8 * | 100 ± 2.1 ** | no | 61 ± 0.6 ** |
| 4 | 86 ± 2.2 ** | 100 ± 3.1 ** | no | 13 ± 0.3 * |
| 5 | 5 ± 0.1 * | no | 29 ± 1.1 ** | 9 ± 0.1 * |
| 6 | 8 ± 0.3 ** | no | no | 9 ± 0.1 * |
| 7 | 2 ± 0.0 * | no | no | no |
| 8 | 9.8 ± 0.4 * | no | no | 7 ± 0.1 * |
| 9 | 41 ± 1.0 ** | 42 ± 1.3 ** | no | 31 ± 0.7 * |
| 10 | 24 ± 0.8 ** | no | 8.5 ± 0.1 ** | 18 ± 0.6 ** |
| 11 | no | no | no | 6 ± 0.1 * |
| 12 | no | no | no | 16 ± 0.1 * |
| 13 | 76 ± 1.1 ** | 87 ± 1.9 ** | 18 ± 0.6 ** | 53 ± 1.2 ** |
| 14 | 95 ± 3.2 ** | 100 ± 2.1 ** | 100 ± 2.5 ** | 100 ± 1.4 ** |
| 15 | no | no | no | 25 ± 1.0 * |
| 16 | 7 ± 0.1 * | 6 ± 0.0 * | 25±1.2 ** | 45 ± 0.9 ** |
| 17 | nt # | nt # | 46 ± 1.0 ** | nt # |
| 18 | nt # | nt # | no | no |
| 19 | no | no | 8 ± 0.3 * | no |
| 20 | 2 ± 0.0 * | 2 ± 0.0 * | no | no |
| MeOH extract | 94 ± 1.9 ** | 100 ± 2.5 ** | 43 ± 0.4 * | 57 ± 1.0 ** |
| MeOH:H2O (5:1) extract | 87 ± 2.1 ** | 8 ± 1.8 ** | 52 ± 0.7 ** | no |
| NDGA | 87 ± 1.1** | 93 ± 1.8 ** | 91 ± 2.3 ** | |
| trolox | 88 ± 0.9 ** |
# Final concentration 0.1mM; no: no activity under the experimental conditions; * p < 0.05; ** p < 0.01; nt #: not tested (The amount of the compounds was very small for the experiments to be performed. Thus, we decided for these compounds to test only their enzyme inhibitory activity for the sake of comparison); significant differences are relative to the solvent control.