Maximum likelihood phylogram of LTP1, LTP2, LTPd and LTPg proteins from the bryophytes M. polymorpha, S. fallax, P. patens, the lycophyte S. moellendorffii, and the spermatophyte A. thaliana. Note that LTP1 and LTP2 are not found in bryophytes or lycophytes. S. fallax LTPs were predicted using KAPPA [73] on genome annotations available at Phytozome v. 12.1 (https://phytozome.jgi.doe.gov/). Other LTPs are from [14]. Amino acid sequences of the 8CM motif were aligned with MUSCLE [74]; the phylogenetic tree was constructed using PhyML 3.0 [75,76] with 100 bootstrap replicates. Bootstrap values over 25 are shown in the figure.