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. 2018 Jan 9;6(1):5. doi: 10.3390/microorganisms6010005

Table 1.

Apparent catalytic constants (Michaelis-Menten constant, turnover number, specific constant) of three fungal oxidases (AAO, GAO) and an unspecific peroxygenase (UPO) for HMF and DFF oxidation at pH 6.0.

Enzyme Substrate KM (mM) kcat (min−1) kcat/KM (M−1 s−1)
AaeUPO HMF 6.07 13,333 36,610
DFF 0.82 1752 35,622
PeryAAO HMF 36.3 219 100
PostAAO HMF 7.2 177 411
GAO HMF 142 42 4.9