Table 2.
FDCA formation from FFCA (2.5 mM) in the presence of different enzymes, enzyme cocktails and effectors. Reactions were performed in phosphate buffer (50 mM, pH 7.25) over 48 h. Concentrations given are mean values of three measurements with standard deviation.
| Reaction Setup | FDCA (mM) |
|---|---|
| AaeUPO | 0.31 ± 0.01 |
| AaeUPO/H2O2 | 0.40 ± 0.05 |
| H2O2 | 0.05 ± 0.01 |
| Hemoglobin/H2O2 | 0.06 ± 0.00 |
| Hemin/H2O2 | 0.06 ± 0.00 |
| AaeUPO/Cat | 1.88 ± 0.11 |
| AaeUPO/SOD | 0.33 ± 0.02 |
| AaeUPO/Cat/SOD | 1.98 ± 0.09 |
| AaeUPO/Cat/SOD/H2O2 | 2.08 ± 0.07 |
| Cat | 1.39 ± 0.09 |
| Cat/SOD | 1.37 ± 0.08 |
| AaeUPO/Cat/GOD | 0.61 ± 0.03 |
| Cat/GOD | 0.08 ± 0.01 |
| HRP | 0.52 ± 0.01 |
| UPO/sodium azide | 0.00 ± 0.00 |
| UPO (boiled) | 0.00 ± 0.00 |
| Cat/sodium azide | 0.00 ± 0.00 |