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. 2017 Dec 25;6(1):2. doi: 10.3390/vaccines6010002

Figure 1.

Figure 1

Cloning Strategy. (A) Sequence of N. lactamica Y92-1009 PorB. The leader sequence is indicated in italic. Loops are indicated as follows: L1: Dark red. L2: Orange. L3: Ochre. L4: Yellow. L5: Lime green. L6: Green. L7: Teal. L8: Blue. Residues in L4, L5, L6 and L7 for insertion of MOMP VDs are in bold underlined red; (B) Cartoon of the NL PorB monomer (side view) created with Swiss-Model server [63,64] and NGL Viewer 0.9.0 [65] based on the Nme PorB template (PDB ID 3WI4) [66]. The loops are color-coded as in (A). (C) Sequence of C. muridarum MOMP. The leader sequence is in italic. Loops are indicated as follows: L1: Underlined. L2: Lime green box with residues corresponding to VD1 bolded. L3: Green box, VD2 residues bolded. L4: Underlined. L5: Teal box, VD3 residues bolded. L6: Yellow box, VD4 residues bolded. Residues in L2, L3, L5 and L6 for insertion into PorB loops are in bold underlined red.