Figure 5.

PorB/VDs induce TLR2 activation and are free of LPS. (A) HEK-blue-hTLR2 cells were incubated with medium alone, purified PorB/VDs and NL PorB (10 μg/mL), Pam₃CSK₄ (100 ng/mL) and TNF-α (50 ng/mL). ^≈ p <0.0001 by one-way ANOVA with Tukey’s multiple comparisons test; (B) HEK-blue hTLR4 cell were incubated with NL PorB and PorB/VDs as in (A), and with E. coli LPS (100 ng/mL). SEAP activity was measured spectrophotometrically at OD₆₂₀ directly in the plate after 14–16 h and is reported as fold-change relative to medium alone ± SEM. ^≈ p < 0.0001 as above; * p < 0.05 by unpaired t test.