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. 2017 Dec 25;6(1):2. doi: 10.3390/vaccines6010002

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PorB/VDs are immunogenic. (A) Total serum IgGs measured by ELISA in sera from mice immunized with NL PorB or PorB/VDs plus CpG DNA and Montanide ISA 720 as adjuvants. PorB/VD1: upward triangles; PorB/VD2: squares; PorB/VD3: downward triangles; PorB/VD4: diamonds; NL PorB: circles. Pre-immune (Pr) and immune sera (week 2, week 4 and week 6) from individual mice in each group were tested in duplicate wells and are expressed as μg/mL ± SEM. ^≈ p < 0.0001 by two-way ANOVA with Tukey’s multiple comparisons test; (B–F) IgG subclasses (μg/mL ± SEM) measured as above. ^≈ p < 0.0003 by one-way ANOVA with Tukey’s multiple comparisons test. *; **; *** p < 0.05 by Mann-Whitney test.