Figure 3.

CRISPR/Cas9-mediated gene editing for creating dystrophin mRNA with a corrected ORF by removing the premature stop codon due to C to T transition shown in red. The dual vector approach targets introns 51 and 53 to direct the excision of exons 52 and 53. The arrows depict the sgRNA target sites in the intronic region shown in 5′-3′ direction based on target strand.