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. 2018 Feb 24;11(1):25. doi: 10.3390/ph11010025

Figure 3.

Figure 3

Schematic representation of Lys-phiSA012 and domain(s) deletion mutants and their lytic activities towards SA003. (A) Schematic representation of the Lys-phiSA012 protein construct. Amino acid residues 29–160: CHAP domain, 188–385: AMID domain, 409–481: SH3b domain. (B) SDS-PAGE of purified Lys-phiSA012 and each domain(s) deletion mutant, performed using 15% polyacrylamide gel. Upper lane; Standard molecular weight markers. (C) Lytic activities of Lys-phiSA012 and each domain(s) deletion mutant towards SA003. Except for ∆mt2, 163.8 pmol of the endolysin was added to the culture medium. For ∆mt2, 45.98 pmol was added.