Table 2.
Plasmids and primers.
| Plasmids | Protein Produced (Amino Acids) | Forward Primes | Reverse Primers | Recipient Vectors |
|---|---|---|---|---|
| pGEX-Lys012WT | 1-495 | Lys012-1Fw | Lys012-495Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt1 | 161-495 | Lys012-161Fw | Lys012-495Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt2 | 1-221, 390-495 | 1-221; Lys012-1Fw | 1-221; Lys012-Δmt2Rv | pGEX-6P-2 |
| 390-495; Lys012-Δmt2Fw | 390-495; Lys012-495Rv | |||
| Overwrap; Lys012-1Fw | Overwrap; Lys012-495Rv | |||
| pGEX-Lys012Δmt2′ | 1-221 | Lys012-1Fw | Lys012-221Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt3 | 1-408 | Lys012-1Fw | Lys012-408Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt4 | 386-495 | Lys012-386Fw | Lys012-495Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt5 | 161-408 | Lys012-161Fw | Lys012-408Rv | pGEX-6P-2 |
| pGEX-Lys012Δmt6 | 1-187 | Lys012-1Fw | Lys012-187Rv | pGEX-6P-2 |
| Primers | Sequences | |||
| Lys012-1Fw | 5′-TCCCCAGGAATTCCCATGGCTAAGACTCAAGCAGA-3′ | |||
| Lys012-161Fw | 5′-TCCCCAGGAATTCCCATGATACCTGTAAAAGCAGGAA-3′ | |||
| Lys012-386Fw | 5′-TCCCCAGGAATTCCCATGACAAGTAGCGCA-3′ | |||
| Lys012-187Rv | 5′-CGCTCCAGTCGACCCCTATTTCTTTTTAGGTGCAG-3′ | |||
| Lys012-221Rv | 5′-CGCTCGAGTCGACCCCTATGAAGAACGACCTGC-3′ | |||
| Lys012-408Rv | 5′-CGCTAGTCGACCCCTAAGTTCCGTACTGGTTC-3′ | |||
| Lys012-495Rv | 5′-CGCTCGAGTCGACCCCTACTTGAATACTCCCCAGG-3′ | |||
| Lys012-Δmt2Fw | 5′-CACAACGATGCAGGTCGTTCTTCAAGTACACCGGCAACTAGACCAGTTAC-3′ | |||
| Lys012-Δmt2Rv | 5′-GTAACTGGTCTAGTTGCCGGTGTACTTGAAGAACGACCTGCATCGTTGTG-3′ | |||
Sequences complementary to linearized pGEX-6P-2 for In-fusion cloning are underlined.