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. 2017 Dec 25;7(1):3. doi: 10.3390/pathogens7010003

Figure 2.

Figure 2

Exogenous PQS restores pyoverdine kinetics in biofilm-defective mutants. (A) Cell aggregate formation in wild-type PA14 or biofilm mutants treated with either DMSO (top) or 100 µM PQS (bottom) after 4 h growth. (B) Pyoverdine fluorescence normalized to bacterial growth measured over 24 h in biofilm mutants treated with DMSO or 100 µM PQS. (C) Biofilm matrix of wild-type (WT) PA14 or c-di-GMP biosynthetic mutants grown in the presence of 100 µM PQS or DMSO solvent for 24 h. Biofilm matrices were stained with 0.1% crystal violet. (D) Quantification of crystal violet stain measured by absorbance at 550 nm after solubilizing in 30% acetic acid solution. (E) Pyoverdine fluorescence, normalized to bacterial growth, measured kinetically over 24 h in c-di-GMP biosynthetic mutants grown in the presence of 100 µM PQS or DMSO solvent. Error bars in (D) represent SEM between three biological replicates. NS corresponds to p > 0.05, # corresponds to p < 0.05, and * corresponds to p < 0.01 based on Student’s t-test. Pyoverdine production curves without normalization to bacterial growth are available in Figure S1.