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Apatite with or without drugs (0 to 100 µM) were formed in bicarbonated medium with addition of 5 and 6 mM of exogenous calcium chloride in presence or absence of drugs, followed by 30 min incubation at 37 °C. After incubation, turbidity was measured at 320 nm against fresh media as a blank. Values for apatite were calculated by deducting values for the free drug at different concentrations. All experiments were performed in duplicate and data represents the mean ± SD.
