Figure 5.

PCR analysis of T₀ transgenic plants containing the Reb and the human lysozyme gene. (A) The plasmid construct, API266 (Native-Reb), the primer positions (indicated by arrows), and amplified fragment size. One primer was designed from the vector region and the other from the Reb terminator. Only transformants can be amplified. (B) The diagram shows construct API264 (Glb-Lys), the primer positions, and the amplified fragment size. The primers hybridize to an internal sequence of the human lysozyme gene. (C) PCR analysis of Native-Reb/Glb-Lys cotransformed plants. Arrows mark the 522-bp fragment of the Reb/vector region and the 278-bp fragment from internal sequence of the human lysozyme gene.