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. 2018 Mar 8;10(1):31. doi: 10.3390/pharmaceutics10010031

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The representative chromatograms of creatine-¹³C (black) and creatine-d₃ (red) in blank plasma (A), a 10 μg/mL standard sample of creatine-¹³C in plasma (B), and an unknown rat plasma sample in similar range as the standard which we measured as 10.7 μg/mL creatine-¹³C (C). The assay was developed for the simultaneous measurement of creatine-guanidino-¹³C and creatine-methyl-d₃ (D). Intensity is in counts per minute (CPM).