Table 2.
Model parameters used in the macroscopic Monod model for the simulation of CHO cultivations based on literature (Lit.) or experimental data (exp). Literature data were determined using fed-batch CHO cell culture and the markov chain monte carlo method for parameter estimation [49] or by the differential evolution technique [50].
| Parameter | Description | Value | Unit | Source |
|---|---|---|---|---|
| µmax | Maximum growth rate | 0.039 | h−1 | exp |
| kd | Maximum death rate | 0.004 | h−1 | exp |
| Kglc | Monod constant glucose | 1.00 | mM | [50] |
| Kgln | Monod constant glutamine | 0.047 | mM | [49] |
| KIlac | Monod constant lactate for inhibition | 43.00 | mM | [49] |
| KIamm | Monod constant ammonium for inhibition | 6.51 | mM | [49] |
| KDlac | Monod constant lactate for death | 45.8 | mM | [49] |
| KDamm | Monod constant ammonium for death | 6.51 | mM | [49] |
| YX/glc | Yield coefficient cell conc./glucose | 0.357 | E9 cells mmol−1 | exp |
| YX/gln | Yield coefficient cell conc./glutamine | 0.974 | E9 cells mmol−1 | [49] |
| Ylac/glc | Yield coefficient lactate/glucose | 0.70 | mmol mmol−1 | exp |
| Yamm/gln | Yield coefficient ammonium/glutamine | 0.67 | mmol mmol−1 | [49] |
| ramm | Ammonium removal rate | 6.3 | E-12 mmol cell−1·h−1 | [49] |
| mglc | Glucose maintenance coefficient | 69.2 | E-12 mmol cell−1·h−1 | [49] |
| a1 | Coefficient for mgln | 3.2 | E-12 mmol cell−1·h−1 | [49] |
| a2 | Coefficient for mgln | 2.1 | mM | [49] |
| QmAb | Specific production rate | 1.51 | E-12 g·c−1·h−1 | exp |