FIG 4 .

IL-1β treatment drives expression of IFN-β and ISGs. (A) Schematic diagram of the microarray design used in this study. WT BMDCs were mock treated or treated with IL-1β (100 ng/ml). Total RNA was extracted at 24 and 48 h posttreatment and subjected to Agilent Whole Mouse Genome Microarray analysis. (B) Gene expression levels were determined as fold changes with respect to matched, mock-treated controls. A significant changes is defined as a >1.5-fold increase or decrease with respect to mock treatment, with a BH-adjusted P value of <0.05. IL-1β-regulated genes were plotted on a heat map with hierarchical clustering by Euclidean distance. Gene clusters are labeled with the most significantly enriched biological process in that group. The abbreviation ns signifies no significantly enriched categories in that cluster. (C) IL-1β-driven genes were compared against genes found to be induced upon IFN-β treatment of WT BMDCs. ISGs regulated by IL-1β as defined for panel B were plotted on a heat map.