FIG 3 .

ADCC responses detected with assays relying on the total cell population. Primary CD4⁺ T cells infected with the NL4.3 ADA GFP virus, either wild-type (HIV WT) (depicted in gray) or defective for Nef and Vpu expression (HIV N⁻ U⁻) (depicted in black), were used as target cells in the granzyme B assay (A to C), the NK cell activation assay (D to F), or FACS-based assays (gating on the total cell population) (G to I). (A, D, and G) ADCC responses detected with the anti-Env MAbs (5 μg/ml) A32, PGT126, and 3BNC117 against cells infected with WT virus. (B, C, E, F, H, and I) ADCC responses mediated by A32 (B, E, and H) or HIV⁺ and HIV⁻ sera (1:1,000 dilution) (C, F, and I) against cells infected with WT or N⁻ U⁻ virus. All graphs shown represent ADCC responses obtained for at least 5 independent experiments. Error bars indicate means ± standard errors of the means. Statistical significance was tested using unpaired t test or Mann-Whitney test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, nonsignificant).