FIG 7 .

Measurement of ADCC responses against gp120-coated target cells. (A and B) Recognition of gp120-coated CEM.NKr cells by A32, PGT126, or 3BNC117 (A) and HIV⁺ or HIV⁻ sera (B). (C and D) ADCC responses detected using the RFADCC assay against gp120-coated cells with the anti-Env Ab A32, PGT126, or 3BNC117 (0.008, 0.04, 0.2, 1, and 5 μg/ml) (C) and HIV⁺ or HIV⁻ sera (1:100, 1:400, 1:1,600, 1:6,400, and 1:25,600 dilutions) (D). All graphs shown represent staining and ADCC responses obtained in at least 3 independent experiments. Error bars indicate means ± standard errors of the means. Statistical significance was tested using unpaired t test (****, P < 0.0001).