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. 2018 Mar 20;9(2):e00358-18. doi: 10.1128/mBio.00358-18

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Copyright © 2018 Richard et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 9 — The CD4⁺ p24⁺ cell population represents a minimal fraction of the gag-pol mRNA⁺ cells in HIV-1-infected individuals. CD4⁺ T cells isolated from chronically HIV-infected, untreated individuals were rested overnight. Cells were then stained for phenotypic markers (see Materials and Methods) prior to detection of HIV-1 p24 and gag-pol mRNA by RNA-flow FISH. (A) Example of flow cytometry gating based on p24 and CD4 expression. (B) Quantification of the percentage of cells positive for gag-pol mRNA based on CD4 and p24 levels. Error bars indicate means ± standard errors of the means of data obtained with 10 HIV-1-infected individuals.