FIG 6.

Infection of HIEC-6 human intestinal epithelial cells with S. Javiana does not induce apoptosis or cell death regardless of S-CDT status. HIEC-6 cells were infected with wild-type or ΔcdtB S. Javiana strains for 48 h or treated with 50 µM H₂O₂ for 8 h (for a positive control). Cells were stained with annexin V conjugated to Alexa Fluor 488 (AF488); PI was used as a live/dead stain. (A) Representative images of density plots of annexin V and PI staining of HIEC-6 cells infected with S. Javiana, treated with H₂O₂, or untreated. (B and C) Quantification of the proportions of PI-positive cells (B) and annexin V-positive cells (C) shown in panel A. Treatments that do not share letters have significantly different (P < 0.05) proportions of cells positive for either annexin V or PI staining. Results in panels B and C are from four independent experiments. Error bars represent standard errors of the means.