Table 2.

Characteristics of peptide fragments of CXCR3.

#	Sequence1	Length	Residues2	MW3	Hydrophobicity4	pI5	Charge6
1	MVLEVSDHQVLNDAEVAALL	20	1–20	2392.3	0.72	3.74	−3.92
2	LNDAEVAALLENFSSSYDYG	20	11–30	2441.0	−0.13	3.30	−4.09
3	ENFSSSYDYGENESDSCCTS	20	21–40	2486.8	−1.26	3.23	−5.18
4	ENESDSCCTSPPCPQDFSLN	20	31–50	2435.7	−0.96	3.30	−4.23
5	PPCPQDFSLNFDRAFLPALY	20	41–60	2536.5	−0.06	4.11	−1.14
6	FDRAFLPALYSLLFLLGLLG	20	51–70	2464.9	1.50	6.33	−0.09
7	SLLFLLGLLGNGAVAAVLLS	20	61–80	2188.8	2.04	6.10	−0.09
8	NGAVAAVLLSRRTALSSTDT	20	71–90	2228.4	0.31	10.40	0.91
9	RRTALSSTDTFLLHLAVADT	20	81–100	2413.6	0.20	7.55	0.08
10	FLLHLAVADTLLVLTLPLWA	20	91–110	2446.1	1.82	5.29	−0.92
11	LLVLTLPLWAVDAAVQWVFG	20	101–120	2476.0	1.63	3.75	−1.09
12	VDAAVQWVFGSGLCKVAGAL	20	111–130	2215.7	1.16	6.16	−0.14
13	SGLCKVAGALFNINFYAGAL	20	121–140	2255.0	1.06	8.52	0.86
14	FNINFYAGALLLACISFDRY	20	131–150	2538.0	0.90	6.16	−0.14
15	LLACISFDRYLNIVHATQLY	20	141–160	2579.6	0.75	7.35	0.03
16	LNIVHATQLYRRGPPARVTL	20	151–170	2500.9	−0.11	12.20	3.07
17	RRGPPARVTLTCLAVWGLCL	20	161–180	2408.2	0.62	10.52	2.81
18	TCLAVWGLCLLFALPDFIFL	20	171–190	2501.5	2.07	3.75	−1.18
19	LFALPDFIFLSAHHDERLNA	20	181–200	2551.7	0.31	5.36	−1.75
20	SAHHDERLNATHCQYNFPQV	20	191–210	2592.7	−1.13	6.78	−0.64
21	THCQYNFPQVGRTALRVLQL	20	201–220	2571.2	−0.20	9.50	2.03
22	GRTALRVLQLVAGFLLPLLV	20	211–230	2375.3	1.50	12.50	1.91
23	VAGFLLPLLVMAYCYAHILA	20	221–240	2404.6	1.93	7.35	0.02
24	MAYCYAHILAVLLVSRGQRR	20	231–250	2546.0	0.51	10.13	3.02
25	VLLVSRGQRRLRAMRLVVVV	20	241–260	2545.8	0.85	13.10	4.91
26	LRAMRLVVVVVVAFALCWTP	20	251–270	2471.0	1.85	10.53	1.86
27	VVAFALCWTPYHLVVLVDIL	20	261–280	2497.5	1.92	5.29	−0.97
28	YHLVVLVDILMDLGALARNC	20	271–290	2454.4	1.21	5.41	−0.97
29	MDLGALARNCGRESRVDVAK	20	281–300	2388.1	−0.36	8.55	0.86
30	GRESRVDVAKSVTSGLGYMH	20	291–310	2374.9	−0.43	9.30	1.08
31	SVTSGLGYMHCCLNPLLYAF	20	301–320	2415.2	0.85	7.25	−0.02
32	CCLNPLLYAFVGVKFRERMW	20	311–330	2672.8	0.50	8.80	1.81
33	VGVKFRERMWMLLLRLGCPN	20	321–340	2644.2	0.25	11.38	2.86
34	MLLLRLGCPNQRGLQRQPSS	20	331–350	2492.9	−0.49	12.20	2.86
35	QRGLQRQPSSSRRDSSWSET	20	341–360	2574.1	−2.01	12.02	1.91
36	SRRDSSWSETSEASYSGL	18	351–368	2229.0	−1.27	4.43	−1.09

Sequence of peptides in one letter format. Each peptide listed is biotinylated at the N-terminus.

Residues are numbered according to Uniprot entry P49682 (CXCR3_HUMAN) isoform 1.

Molecular weight as determined by mass spectrometric quality report of manufacturer.

Hydrophobicity scores were calculated with the function hydrophobicity of R package peptides by the method of Kyte and Doolittle (21).

pI (isoelectric point) values were calculated with the function pI of R package peptides with the default EMBOSS method.

The peptide sum charge was calculated a pH 7.2 with the function charge of R packages peptides, with the method Stryer.