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. Author manuscript; available in PMC: 2018 Mar 29.
Published in final edited form as: Yeast. 2009 Aug;26(8):451–463. doi: 10.1002/yea.1678

Figure 5.

Figure 5

RSM is enhanced by M16A proteases. (A) Axl1p and Ste23p were independently overexpressed in yWS164 in the presence of the a-factor CKQQ variant and the strains were subjected to a serial dilution mating test, as described in Figure 2. The first two dilution spots are shown. The plasmids used were pRS316, pWS601, pWS602 and pWS727. (B) The ability of overexpressed Ste23p to enhance RSM was evaluated for CaaX motifs that were previously identified as either not promoting (e.g. CVIA and CDQQ) or promoting RSM (e.g. CKQQ). The plasmids used were pWS602, pWS610, pWS727 and pWS853. The experiments shown in (A, B) were performed on separate days and a slight variation in mating efficiency is apparent for the same strain, which is labelled STE23 in (A) and CKQQ in (B)