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Semiquantification of hemosiderin, lipofuscin, and 8-OHdG in aged ovaries. Percentages of positive signal areas of (a) Perls, n = 8 per group, and (b) SBB, n = 7 per group, relative to the total ovarian area as extracted with the ImageJ1 software. (c) 8-OHdG in isolated genomic DNA, n = 6 per group; AU = chemiluminescent arbitrary units measured with the Image Studio Digits software (for details, see Materials and Methods). ∗∗p < 0.005, nonparametric Mann–Whitney test.
