Table 4.
Plasmids and strains used in this study
| Plasmids and strains | Description | Source |
|---|---|---|
| Plasmids | ||
| pETDuet1 | Amp r oriP BR322 lacI q P T7 | Novagen |
| pACYCDuet1 | Cm r oriP 15A lacI q P T7 | Novagen |
| pLMe1 | pETDuet1-ghrA | This study |
| pLMe2 | pETDuet1-yjhH-xylC-xdh | This study |
| pLMe3 | pETDuet1-yjhH-xylC-ghrA-xdh | This study |
| pLMa1 | pACYCDuet1-aceA | This study |
| pLMa2 | pACYCDuet1-aldA-yjhG | This study |
| pLMa3 | pACYCDuet1-aldA-aceA-yjhG | This study |
| Strains | ||
| E. coli DH5α | F− supE44 ΔlacU169 (ϕ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| E. coli BL21(DE3) | F− ompT gal dcm lon hsdSB (rB− mB−) λ(DE3) | Invitrogen |
| JW3974 | E. coli BW25113 aceB::kan | [39] |
| JW2946 | E. coli BW25113 glcD::kan | [39] |
| JW3978 | E. coli BW25113 iclR::kan | [39] |
| JW2293 | E. coli BW25113 ackA::kan | [39] |
| Q2589 | E. coli BL21(DE3) aceB::kan | This study |
| Q2723 | E. coli BL21(DE3) glcD::kan | This study |
| Q2280 | E. coli BL21(DE3) iclR::kan | This study |
| Q1951 | E. coli BL21(DE3) ackA::kan | This study |
| Q2728 | E. coli BL21(DE3)/pLMe1/pLMa1 | This study |
| Q2705 | E. coli BL21(DE3) aceB::kan/pLMe1/pLMa1 | This study |
| Q2562 | E. coli BL21(DE3)/pLMe2/pLMa2 | This study |
| Q2590 | E. coli BL21(DE3) aceB::kan/pLMe2/pLMa2 | This study |
| Q2725 | E. coli BL21(DE3)/pLMe2/pLMa3 | This study |
| Q2726 | E. coli BL21(DE3)/pLMe3/pLMa2 | This study |
| Q2727 | E. coli BL21(DE3) glcD::kan/pLMe2/pLMa2 | This study |
| Q2729 | E. coli BL21(DE3)/pLMe3/pLMa3 | This study |
| Q2741 | E. coli BL21(DE3) iclR::kan/pLMe2/pLMa2 | This study |
| Q2742 | E. coli BL21(DE3) ackA::kan/pLMe2/pLMa2 | This study |