Table 3.

Selective CRISPR/Cas9 plasmids

	Name	Gene/insert	Selectable marker	Purpose	Reference
1.CRISPR/Cas9 plasmids that create single stranded break (Mammalian)	pX335-U6-chimeric_BB-CBh-hSpCas9n (D10A)	Humanized S. pyogenes Cas9(D10A) nickase	None	A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid	[28]
2.CRISPR/Cas9 plasmids that create double stranded break (Mammalian)	pX330-U6-chimeric_BB-CBh-hSpCas9	humanized S. pyogenes Cas9	None	A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid	[28]
	pSpCas9 (BB)-2A–Puro (PX459) V2.0	hSpCas9-2A-Puro V2.0 (Synthetic)	Puromycin	Cas9 from S. pyogenes with 2A–Puro, and cloning backbone for sgRNA (V2.0)	[31]
3.CRISPR/Cas9 plasmids which activate gene expression(Mammalian)	SP-dCas9-VPR	SP-dCas9-VPR (Homo sapiens)	Neomycin (select with G418)	SP-dCas9 with VP64-p65-Rta (VPR) fused to its C-terminus; mammalian vector	[33]
	dCAS9-VP64_GFP	dCAS9(D10A, H840A)-VP64_2A_GFP (Synthetic)	GFP	Expresses dCas9-VP64 activator with 2A GFP	[34]
	lenti dCAS-VP64_Blast	dCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic)	Blasticidin	3rd generation lenti vector encoding dCas9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)	[34]
4.CRISPR/Cas9 plasmids which repress gene expression(Mammalian)	pSLQ1658-dCas9-EGFP	dCas9 fuse to EGFP(Homo sapiens)	Puromycin	Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can beTetON 3G promoter system)	[35]
	pLV hUbC-dCas9-T2A-GFP	Humanized dead Cas9T2A GFP (Other)	Zeocin	Co-expresses human optimized S. pyogenes dCas9 and GFP	[36]
	pJMP1	dCas9 (Other)	None	Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA	[37]
5.CRISPR/Cas9 plasmid for tagging protein	Protein pFETch_NCoA2	Human	Donor plasmid pFETCh_NCoA2	Donor vector for 3’ FLAG tag of human NCoA2	[38]
	pFETch_RAD21	Human	pFETCh_RAD21	Homology arms and 3X Flag with P2A Neofor 3′ tagging of human RAD21	[38]