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Sequences of the primers of the target and housekeeping genes used for RT-PCR.
| Sequence (5′-3′) | Length of product | |
|---|---|---|
| GAPDH | Sense primer: 5-GGCCTTCCGTGTTCCTACC-3 | 103 bp |
| Antisense primer: 5-TGCCTGCTTCACCACCTTC-3 | ||
| RUNX2 | Sense primer: 5-CCAAGTAGCCAGGTTCAACG-3 | 64 bp |
| Antisense primer: 5-GGTGAAACTCTTGCCTCGTC-3 | ||
| OSX | Sense primer: 5-CTTTCGTCTGCAACTGGCTT-3 | 123 bp |
| Antisense primer: 5-TAAAGCGCTTGGAACAGAGC-3 | ||
| OPN | Sense primer: 5-CAGCCATGAGTCAAGTCAGC-3 | 116 bp |
| Antisense primer: 5-TGTGGCTGTGAAACTTGTGG-3 |
