Table 1.
Sense and antisense primer sequences used for RT-PCR.
| Name | Sense | Antisense |
|---|---|---|
| Occludin | CCTCCAATGGCAAAGTGAAT | CCCCACCTGTCGTGTAGTCT |
| Claudin 5 | GCTCTCAGAGTCCGTTGACC | ATCTAGTGCCCCCAGGATCT |
| TJP-1 gene (ZO-1 protein) | ACCCAGCAAAGGTGTACAGG | CCGTAGGCGATGGTCATAGT |
| VE-cadherin | CAATGACAACTTCCCCGTCT | CGTTTGGGGTCTGTCTCAAT |
| Angiopoietin 1 | GGTCAACAGAATCGCCACTT | CCTGTTCCCATTTGCTGTTT |
| Angiopoietin 2 | AATGTTCCGTGGGAGTTCAG | AACCTGTGCCCACCACTTAG |
| Tie-2 (or TEK) | TCTGGGTGGCCACTACCTAC | TGAAAGGCTTTTCCACCATC |
| eNOS | AAGCTGCAGGTATTTGATGC | TATAGCCCGCATAGC |
| VEGF-A | CCTTAATCCAGAAAGCCTGACATG | AAAGTGCTCCTCGAAGAGTCTCC |
| VEGFR-2 | GGCGGTGGTGACAGTATCTT | GTCACTGACAGAGGCGATGA |
| PDGF-B | GGCCACACACCTTCTCTGAT | GTGGAGGAGCAGACTGAAGG |
| PDGFR-1 | CACCTTCTCCAGTGTGCTGA | GGAGTCCATAGGGAGGAAGC |
| TGF-β (1) | TTGCTTCAGCTCCACAGAGA | TGGTTGTAGAGGGCAAGGAC |
| TGFR-2 | GCAAGTTTTGCGATGTGAGA | GGCATCTTCCAGAGTGAAGC |
| Interleukin-1β | ACCTTCCAGGATGAGGACATGA | AACGTCACACACCAGCAGGTTA |
| Interleukin-6 | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC |
| Interferon-γ | GCTTTGCAGCTCTTCCTCAT | GTCACCATCCTTTTGCCAGT |
| TNF-α | TGGCCTCCCTCTCATCAGTTC | TTGGTGGTTTGCTACGACGTG |
| MCP-1 (or CCL2) | AGGTCCCTGTCATGCTTCTG | TCTGGACCCATTCCTTCTTG |
TJP-1: tight junction protein 1; ZO-1: zona occludens-1; VE-cadherin: vascular endothelial-cadherin; eNOS: endothelial nitric oxide synthase; VEGF-a: vascular endothelial growth factor-a; VEGF-R2: vascular endothelial growth factor receptor 2; PDGF-B: platelet-derived growth factor-B; PDGFR-1: platelet-derived growth factor receptor-1; TGF-β: transforming growth factor-β; TGFR-2: transforming growth factor-β receptor-2; TNF-α: tumor necrosis factor-α; MCP-1: monocyte chemoattractant protein-1.