Table 2.

PCR assays targeting the Francisella Outer Membrane Protein A used for F. tularensis ssp. screening.

Target		Sequence	Primer length
FopA-F	Forward primer	5′-CAAATCTAGCAGGTCAAGCAACAG-3′	24
FopA-R	Reverse primer	5′-CACTTGCTTGAACATTTCTAGATAGTTCA-3′	29
FopA-S	Probe	FAM-5′-TGCTTGGGATGTGGGTGGTGGTC-3′-BHQ1	23

Amplicon length = 270 bp.