Figure 3.

Iohexol (240 mg/mL; 25, 50, 100, or 200 µL) was injected intramuscularly into the caudal thigh muscles of mice, and tissue distribution was evaluated over time (10 through 180 min after injection). In the (A) 25-µL and (B) 50-µL injection groups, intramuscularly injected iohexol appears to remain exclusively in the intramuscular space throughout the study. In the (C) 100-µL and (D) 200-µL injection groups, a considerable portion of the intramuscularly injected iohexol appears to leak into the adjacent, extramuscular space over time. Representative animals from each volume group are shown.